![]() Hyalocytes: essential cells of the vitreous cavity in vitreoretinal pathophysiology? Retina 31, 222–228 (2011). Prdm proto-oncogene transcription factor family expression and interaction with the Notch-Hes pathway in mouse neurogenesis. Deep learning and alignment of spatially-resolved whole transcriptomes of single cells in the mouse brain with Tangram. Hybridization-based in situ sequencing (HybISS) for spatially resolved transcriptomics in human and mouse brain tissue. Sequence and expression analysis of Nhlh1: a basic helix-loop-helix gene implicated in neurogenesis. N., Eddleston, J., Leblond-Bourget, N., Stanier, P. Genome-wide atlas of gene expression in the adult mouse brain. Single-cell RNA sequencing of microglia throughout the mouse lifespan and in the injured brain reveals complex cell-state changes. Single-cell transcriptomic analyses of the developing meninges reveal meningeal fibroblast diversity and function. Regulation of Six1 expression by evolutionarily conserved enhancers in tetrapods. ![]() Pax2/5 and Pa圆 subdivide the early neural tube into three domains. En1 and Wnt signaling in midbrain dopaminergic neuronal development. Pa圆 transcription factor is required for the interkinetic nuclear movement of neuroepithelial cells. A single-cell molecular map of mouse gastrulation and early organogenesis. Hes genes regulate size, shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation. Pa圆 is a human neuroectoderm cell fate determinant. Location of transient ectodermal progenitor potential in mouse development. Foxa2 regulates polarity and epithelialization in the endoderm germ layer of the mouse embryo. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. ![]() in Patterning and Cell Type Specification in the Developing CNS and PNS (eds. The single-cell transcriptional landscape of mammalian organogenesis. Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding. Molecular architecture of the mouse nervous system. A single-cell transcriptional atlas of the developing murine cerebellum. Molecular diversity of midbrain development in mouse, human, and stem cells. Temporal patterning of apical progenitors and their daughter neurons in the developing neocortex. A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex. ![]() Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex. Molecular identity of human outer radial glia during cortical development. Human cerebral organoids recapitulate gene expression programs of fetal neocortex development. Integrating the in situ data with our single-cell clusters revealed the precise spatial organization of neural progenitors during the patterning of the nervous system.Ĭamp, J. We also used in situ mRNA sequencing to map the spatial expression patterns of key developmental genes. We identified almost eight hundred cellular states that describe a developmental program for the functional elements of the brain and its enclosing membranes, including the early neuroepithelium, region-specific secondary organizers, and both neurogenic and gliogenic progenitors. Here we report a comprehensive single-cell transcriptomic atlas of the embryonic mouse brain between gastrulation and birth. Previous studies have explored development in specific brain regions 1, 2, 3, 4, 5, 6, 7, 8, the whole adult brain 9 and even entire embryos 10. The ability of single-cell RNA sequencing and spatial transcriptomics to reveal the molecular heterogeneity of complex tissues has therefore been particularly powerful in the nervous system. A complete understanding of this process requires a systematic characterization of cell states over the entire spatiotemporal range of brain development. ![]() The mammalian brain develops through a complex interplay of spatial cues generated by diffusible morphogens, cell–cell interactions and intrinsic genetic programs that result in probably more than a thousand distinct cell types. ![]()
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